========================= Welcome to Limelight ========================= **Limelight** is a web application for analyzing, visualizing, and sharing and bottom-up proteomics results. Citing =============== If you use Limelight in your work, please cite: .. epigraph:: `Limelight: An Open, Web-Based Tool for Visualizing, Sharing, and Analyzing Mass Spectrometry Data from DDA Pipelines. Riffle M, Zelter A, Jaschob D, Hoopmann MR, Faivre DA, Moritz RL, Davis TN, MacCoss MJ, Isoherranen N. J Proteome Res. 2025 Apr 4;24(4):1895-1906. doi: 10.1021/acs.jproteome.4c00968. `_ Getting Help, Providing Feedback, or Reporting Problems ======================================================= If after reading this documentation you have questions about Limelight, ideas for new features, or want to report any problems, please contact us! * Try using the `Limelight Issue Page at GitHub `_. * You can `join our Slack `_ and chat with us. * You can email us at limelightms@uw.edu Introduction ============= **Limelight** is designed to provide you with the full-stack of proteomics results, regardless of which processing pipeline you used to search your data. Full-stack means that you have access to the global views of your data (such as statistically comparing conditions), to viewing lists of proteins and peptides, to individual PSMs and spectra--all showing the native scores from whichever pipeline you used. Additionally, all native scores from your pipeline are available to you for filtering--even when contrasting multiple searches that each used different pipelines. Tutorials for Running Pipeline ================================== We have developed tutorials to accompany Riffle M, Hoopmann M, et al. (2022) that describe the process for running the Magnum, Percolator, Limelight pipeline; and how to then perform the statistical test for finding mods of interest in Limelight. Please see our :ref:`Tutorials Page ` .. toctree:: :maxdepth: 2 self using-limelight tutorials administration